Comparison of antibody titers using conventional tube technique versus column agglutination technique in ABO blood group incompatible renal transplant

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Comparison of antibody titers using conventional tube technique versus column agglutination technique in ABO blood group incompatible renal transplant

INTRODUCTION Measurement of alloantibody titer to a red cell antigen (ABO titers) is an integral part of management of ABO incompatible kidney transplants (ABOiKT). MATERIAL AND METHODS There are different methods of titer estimation. Alloantibody detection by tube titration and Gel agglutination columns are accepted methodologies. It is essential to find the difference in titers between the ...

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Comparison of Total and IgG ABO Antibody Titers in Healthy Individuals by Using Tube and Column Agglutination Techniques

BACKGROUND Most immune reactions related to transfusion and transplantation are caused by IgM ABO antibodies. However, IgG also plays an important role in these reactions. Therefore, a method to measure antibodies, including IgG, is necessary. We investigated ABO antibody titers of healthy individuals using a column agglutination technique (CAT) with or without dithiothreitol (DTT) and compared...

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Reusing Immunoadsorption Column – Making the ABO Incompatible Renal Transplant Affordable

© 2017 Indian Journal of Nephrology | Published by Wolters Kluwer Medknow Sir, A 47‐year‐old male, known case of chronic kidney disease Stage 5, type 2 diabetes mellitus and hypertension on maintenance hemodialysis for 1 year presented for renal transplant. He had been treated for hepatitis C infection and was in remission. The prospective donor was his wife. Patient’s blood group was O positiv...

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Conventional tube agglutination with polyethylene glycol versus Red Cell Affinity Column Technology (ReACT): a comparison of antibody detection methods.

A procedure for antibody detection and identification that utilizes affinity microcolumns to isolate IgG antibodies in a gel matrix containing Protein G and Protein A was commercially available in recent years. We evaluated this method (ReACT, Red Cell Affinity Column Technology, Immucor Co., Norcross, GA) as an alternative to standard tube agglutination testing, in an effort to minimize subjec...

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ژورنال

عنوان ژورنال: Asian Journal of Transfusion Science

سال: 2017

ISSN: 0973-6247

DOI: 10.4103/0973-6247.214343